Alexanian A.R, Epigenetic modulators promote mesenchymal stem Cell phenotype switches. The International Journal of Biochemistry & Cell Biology, 2015, 64: 190–194.

   The mission of Cell Reprogramming & Therapeutics LLC is the development of cell reprogramming solutions for generation of neuronal and glial subtypes that could potentially be used as cellular models for drug discovery and toxicity studies, as well as cell-based therapeutics for several neurological disorders.  The small molecule approach (synonyms are chemical or chemical genetics approach) is the main strategy for reprogramming adult somatic cells into neural cell types. The main tools are epigenetic modifiers and modulators of specific cell-signaling pathways which we have been using to develop unique cell reprogramming cocktails for production of neural cells.
   In addition, we aim to provide scientists with the most recent news in the field of cell reprogramming and to supply them with small molecule cell reprogramming chemicals individually or in combinations as kits.      


Cell Reprogramming & Neural Regeneration


   The field of neural regeneration has been growing exponentially in the last two decades.  Previously, it was generally held that there was virtually no regeneration or possibility for regeneration in the Central Nervous System (CNS).  This notion has changed as recent studies have demonstrated that CNS regeneration and repair are indeed possible. Our main goal is to run research projects aiming to advance the field of neural repair and to develop new technologies to enhance regeneration after injury or neurodegeneration. 

   Recent cell  reprogramming studies indicate that mammalian somatic cells have the potential to achieve pluripotent states and undergo cell type switching. Such cellular traits are observed under natural conditions in animals that regenerate complex organs. Elucidating the mechanisms that underlie these processes may open new therapeutic avenues for regenerative medicine. The prize would be a paradigm shift that could fundamentally transform the practice of medicine and healthcare economics.

Epigenetic Editing: towards reprogramming of gene expression

​Alexanian A.R., Liu, Q.,  Zhang, Z . Enhancing the efficiency of direct reprogramming of human mesenchymal stem cells into mature neuronal-like cells with the combination of small molecule modulators of chromatin modifying enzymes, SMAD signaling and cyclic adenosine monophosphate levels. The International Journal of Biochemistry & Cell Biology, 2013; 45: 1633-8.


Zhu S, Wei W, Ding S. Chemical strategies for stem cell biology and regenerative medicine.  Annu Rev Biomed Eng. 2011, 13, 73-90.

Mission

Technology Innovation Center
​10437 ​Innovation Drive, Suite 321,

Wauwatosa, WI 53226-4815

Work: (414) 377-3873

Cell:    (414) 238-5067
Fax:    (414) 778-1178
Email:aalexan@cellrtherapeutics.com
E-mail: info@cellrtherapeutics.com

Relevant Reading

Hou, P., Li, Y., Zhang, X., et.al.  Pluripotent Stem Cells Induced from Mouse Somatic Cells by Small-Molecule Compounds, Science. 2014, 341, 651-654.


John Gurdon , Stem Cells and Prospects of Cell Replacement 

Shinya Ywamanaka: 2014 Breakthrough Prize in Life Sciences Symposium

Sheng Ding, A Chemical Approach to Controlling Cell Fate, Gladsonte Instiute 

LINKS TO RELEVANT VIDEOS

​​Pasque V, Jullien J, Miyamoto K, Halley-Stott RP, Gurdon JB. Epigenetic factors influencing resistance to nuclear reprogramming. Trends Genet. 2011, 12, 516-25.